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Convert image to ND2 file

Following command line tool allows you to convert single image into ND2 file:

limnd2-convert-file-to-nd2 <arguments>

Arguments

Important

Arguments highlighted in bold (input) are required.

  • input

    Input image file to be converted.

  • output

    File name of the converted .nd2 file. If not specified, script will use same name as input file with .nd2 extension.

  • -f

    Force overwrite of the output file if it already exists.

  • --unknown_dimension <dimension>

    Specify which dimension to use if there is an unknown dimension in the input file (e.g., multipage TIFF). Choices: multipoint, timeloop, zstack. Default: multipoint.

Examples

Here are some example usages of the limnd2-convert-file-to-nd2 command:

Convert a single image to ND2 
limnd2-convert-file-to-nd2 ./image.tif
Convert a single image and specify the output file name 
limnd2-convert-file-to-nd2 ./images/image.png ./output/output.nd2
Convert an image and force overwrite if output exists 
limnd2-convert-file-to-nd2 ./images/image.tif -f
Convert an image and specify how to handle unknown dimensions (for example multipage TIFF) 
limnd2-convert-file-to-nd2 ./images/image.tif --unknown_dimension zstack